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This is very much a bug that should be fixed.
Your code is fine, I’ve tested it. It’s erroring out because something else your code is processing isn’t what you expect. Make sure that you are running your code against a copy of the site as it is currently and what you expect it to be.
Role of basic fibroblast growth factor in human trophoblast cell proliferation and migration.
During placentation, trophoblasts establish an invasive platform for the exchange of nutrients and metabolites, so that they may be vital for fetal growth. Human basic fibroblast growth factor (bFGF) exerts important functions in a variety of tissues during embryonic development. In the present study, we used a chemotaxis assay to study the role of bFGF on human trophoblast migration and proliferation in a cell-based, in vitro model of placenta. TCT-H187 human choriocarcinoma cells (TF-1 and JEG-3 trophoblast cell lines) were cultured and exposed to bFGF in a concentration range of 0.0-500.0 ng/mL for 16 hours. The number of migrated cells was determined by the Boyden chamber assay, and the proliferation of treated cells was assessed by a modified 3H-thymidine incorporation assay. The effect of bFGF on cell migration of trophoblasts was dose dependent, with optimum effect at 25 ng/mL. The number of migrated trophoblasts into the bottom chamber was significantly increased by 25.0 ng/mL bFGF (p , and then we just
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