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Effects of Aerosolized GK1.5 on M2 Macrophages and IL-4 in a Murine Model of Type 2 Diabetes Mellitus.
Aerosolized GK1.5 prevents lung inflammation in a murine model of allergic airway disease; however, whether it may be effective for the treatment of pulmonary pathology in type 2 diabetes mellitus (T2DM) is not known. The aims of this study were to investigate the effects of aerosolized GK1.5 on diabetes-related pulmonary immunopathology in a murine model of T2DM. A murine model of T2DM was generated by inducing obesity by feeding mice a high-fat diet (45 kcal/g) over an 8-week period. Aerosolized GK1.5 treatment was initiated at 4 weeks, and treatment continued for 12 weeks. After week 12, the mice were sacrificed and bronchoalveolar lavage fluid and blood were collected. The levels of IL-4 and M2 macrophages were measured in bronchoalveolar lavage fluid. Additionally, lung macrophages were isolated and subjected to flow cytometry, and the expression of M2 markers in macrophages was determined using a flow cytometry assay. The results of a histological assessment indicated that aerosolized GK1.5 inhibited pulmonary inflammation, including the recruitment of M2 macrophages. In addition, aerosolized GK1.5 treatment of mice with T2DM resulted in decreased levels of IL-4, a key cytokine in the pathogenesis of T2DM. Therefore, aerosolized GK1.5 treatment attenuated inflammation in the lung by downregulating IL-4 production and the release of M2 macrophages in mice with T2DM.Anyone in the
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